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ythdf3 antibody (ip)  (ABclonal Biotechnology)

 
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    Structured Review

    ABclonal Biotechnology ythdf3 antibody (ip)
    FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
    Ythdf3 Antibody (Ip), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ythdf3 antibody (ip)/product/ABclonal Biotechnology
    Average 90 stars, based on 1 article reviews
    ythdf3 antibody (ip) - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Overexpression of FTO inhibits excessive proliferation and promotes the apoptosis of human glomerular mesangial cells by alleviating FOXO6 m6A modification via YTHDF3-dependent mechanisms"

    Article Title: Overexpression of FTO inhibits excessive proliferation and promotes the apoptosis of human glomerular mesangial cells by alleviating FOXO6 m6A modification via YTHDF3-dependent mechanisms

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2023.1260300

    FTO regulates FOXO6 m6A modification via YTHDF3-dependent manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
    Figure Legend Snippet: FTO regulates FOXO6 m6A modification via YTHDF3-dependent manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .

    Techniques Used: Modification, Over Expression, Knockdown, Methylation, Mutagenesis, Expressing, Western Blot, Control, Binding Assay, Quantitative RT-PCR

    The binding relationship between YTHDF3 and FOXO6 predicted by RM2Target website.
    Figure Legend Snippet: The binding relationship between YTHDF3 and FOXO6 predicted by RM2Target website.

    Techniques Used: Binding Assay, Modification



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    ythdf3 antibody (ip)  (ABclonal Biotechnology)
    90
    ABclonal Biotechnology ythdf3 antibody (ip)
    FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
    Ythdf3 Antibody (Ip), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ythdf3 antibody (ip)/product/ABclonal Biotechnology
    Average 90 stars, based on 1 article reviews
    ythdf3 antibody (ip) - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    FTO regulates FOXO6 m6A modification via YTHDF3-dependent manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .

    Journal: Frontiers in Pharmacology

    Article Title: Overexpression of FTO inhibits excessive proliferation and promotes the apoptosis of human glomerular mesangial cells by alleviating FOXO6 m6A modification via YTHDF3-dependent mechanisms

    doi: 10.3389/fphar.2023.1260300

    Figure Lengend Snippet: FTO regulates FOXO6 m6A modification via YTHDF3-dependent manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .

    Article Snippet: HGMC lysate samples were incubated with either YTHDF3 antibody (IP) (0202730101, ABclonal, Wuhan, China) or control IgG antibody (IgG) at 4°C for 16 h. Finally, total RNA was extracted and analysed using RT-qPCR assay.

    Techniques: Modification, Over Expression, Knockdown, Methylation, Mutagenesis, Expressing, Western Blot, Control, Binding Assay, Quantitative RT-PCR

    The binding relationship between YTHDF3 and FOXO6 predicted by RM2Target website.

    Journal: Frontiers in Pharmacology

    Article Title: Overexpression of FTO inhibits excessive proliferation and promotes the apoptosis of human glomerular mesangial cells by alleviating FOXO6 m6A modification via YTHDF3-dependent mechanisms

    doi: 10.3389/fphar.2023.1260300

    Figure Lengend Snippet: The binding relationship between YTHDF3 and FOXO6 predicted by RM2Target website.

    Article Snippet: HGMC lysate samples were incubated with either YTHDF3 antibody (IP) (0202730101, ABclonal, Wuhan, China) or control IgG antibody (IgG) at 4°C for 16 h. Finally, total RNA was extracted and analysed using RT-qPCR assay.

    Techniques: Binding Assay, Modification